cbc医学上是什么意思介绍如下:
Cbc means medically as follows:
全血细胞计数( 英文:complete blood count,CBC) 。全血细胞计数是一项筛选性试验,它众多的疾病在诊断都需要用到这项检查。通过那施合练氧证洲频这项试验,医生可以观察到血细胞的增多、减360问答少、被破坏等情况,从而了解到炎症、过敏、血凝革皇雨响政张值阻概差等众多情况,对于疾病的诊断与治疗有着极其重要的作用。
The full blood cell count (
检查过程:
Inspection process:
(1) 制备细胞悬液 对于悬液培养的细胞,可直接进行下面的步骤2(计数与计算过程)快怎喜容件。如果计数对象为贴壁生长的细胞,首先需将培养物制备成细胞悬液。
(1) Preparation of cell suspensions, direct to the next step 2 (counting and calculating) . If the number of objects is a walled cell, the culture must first be prepared as a cell suspension
① 终止培养,将培养液吸出,用PBS洗培养物一次。
1 Discontinuing culture, sucking out the culture fluid with PBS baptism 1
② 给培养瓶内加入1ml 0.25%胰蛋白酶溶液,于37℃消化3-5min 。期间不断在镜下观察。当细胞变圆接近脱壁时,弃消化液。
2 Adds 1ml 0.25% insulin enzyme soluble fluid to digest 3 -5min at 37 °C. During this period, is constantly observed under the lens. When cells change towards desolation, the digestion fluid is discarded.
③ 加入一定致都通本内状适量的培养液(如果这些培养细胞不再有用,可加PBS),用吸管吹打,使细胞脱壁而制成细胞悬液。
3 Add into cell suspensions.
(2) 计数与计算过程
(2) Counting and calculation process
① 在细胞计数板中央放置计数专用的盖玻片。
1 Placing a special cytographic plate in the centre of the cell count plate.
② 用适玻璃虹吸管吸取细胞,让虹吸管在盖玻片上或下侧的计数板凹糟处流出悬液,至盖玻片被液体充满为止。
2 Use glass rainbow straw, so that the dysentery of the dysenters on or on the lower side of the lid will flow the suspension until the lid
③ 置显微镜下计数告庆奏右题系底九四角大方格内的细胞总数。
3 The number of cells in the four-channel square at the bottom of the right theme is
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